Version
TNT version and version 1.1 (July 2007) in Hippu and Murska.
Description
TNT is a very fast program for the inference of phylogenies with parsimony method.
Usage
For TNT a NEXUS-formatted sequence alignment file is needed.
On Hippu, the program is started with the command tnt. On Murska user should type the command /v/linux26_x86_64/appl/molbio/tnt/tnt-init.sh in the home directory before using the program.
After initialization the program can be run using a command tnt. Commands are types in the program's own prompt (tnt*>).
A simple analysis using TNT could proceed as follows. Note the placing of semicolons!
1. Read in the data: p rat1.nxs
2. Switch taxon names ON: taxname =
3. Set outgroup taxon: out 12
4. Hold a maximum of 100 trees in memory: hold 100
5. Tree search using 100 RAS and TBR: mult=tbr replic 100 hold 1;
6. Display tree lengths: length;
7. Save the trees in parenthetical format: tsave *trees.out; save; tsave /;
8. Resampling using jackknifing: resample=jak probability 34 frequency;
9. Quitting the program: quit;
Manual
The manual gives background to the implementation features. A detailed description of TNT commands is found in the command manual. TNT also comes with a built-in help. You can get help for individual commands by typing, e.g., help procedure (help and the name of the command).
Manuals for Hennig86 and Paup* might be of use, also.
You might also want to read the worked-out examples in Molecular systematics - material for practicals".
Distribution
TNT can be downloaded and purchased from it's website.
User support
| Saren Ari-Matti | +358 9 457 2282 | Ari-Matti.Saren at csc.fi |