NGS Assembly Course - Training
|Date:||06.09.2012 9:00 - 06.09.2012 17:00|
|lecturers:|| Panu Somervuo |
Metapopulation Research Group & DNA Sequencing and Genomics Lab
University of Helsinki
Topics of this course are genome and transcriptome denovo assembly.
Participants should have basic skills of Linux since all tools are based on command line interface.
Course consists of lectures and hands on exercises. Used tools include Velvet, Trinity, Oases, SOAPdenovo, Minimo (AMOS).
09:00 Introduction: genome projects, NGS data, denovo assembly methods
10:15 Genome Assembly I (velvet)
13:00 Genome Assembly II (SOAPdenovo)
14:00 Combining Assemblies (amos Minimo)
15:15 RNA-Seq, Transcriptome Assembly (velvet+oases, Trinity)
Material for the exercises